Microbiology

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Pyrophosphate [PPi]

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Microbiology

Definition

Pyrophosphate (PPi) is a byproduct of many biochemical reactions, particularly those involving ATP hydrolysis. It plays a crucial role in energy metabolism and enzyme regulation.

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5 Must Know Facts For Your Next Test

  1. PPi is formed when ATP is hydrolyzed to AMP.
  2. It acts as an inhibitor for some enzymes, preventing overactivity.
  3. Inorganic pyrophosphatase catalyzes the hydrolysis of PPi into two orthophosphates (Pi).
  4. PPi can serve as an energy source in certain microbial metabolic pathways.
  5. Its accumulation can affect the balance of cellular phosphate levels, impacting microbial growth.

Review Questions

  • What reaction typically produces pyrophosphate (PPi) in cellular metabolism?
  • How does inorganic pyrophosphatase function in relation to PPi?
  • Why is the regulation of PPi levels important for microbial cells?

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